Disc One:
- Widescreen Presentation
- English and French 5.1 (Dolby Digital)
- Subtitles: English, French, Spanish
- Weaving the Web: Subtitled Factoids
- Commentary: Sam Raimi (director), Kirsten Dunst, Laura Ziskin (producer) & Grant Curtis (co-producer)
- SFX Commentary: John Dykstra (special effects designer) & Visual Effects Crew
- Branching Web-i-sodes
- Music Videos: Chad Kroeger featuring Josey Scott: Hero & Sum 41: What We're All About
- TV Spots
- Theatrical Trailers
- Filmographies & Character Files
- DVD-ROM: Comic/Feature Comparison; Record Your Own Commentary; Countdown to Spider-Man 2; Weblinks

Disc Two:
- HBO Making of Spider-Man
- Spider-Mania: An E! Entertainment Special
- Director Profile: Sam Raimi
- Composer Profile: Danny Elfman
- Screen Tests: Tobey Maguire, J.K. Simmons & CGI Spider-Man
- Costume and Makeup Tests
- Gag/Outtake Reel
- Conceptual Art & Production Design Gallery


雖然韓版發行了
美版也要跟進嚕